yvdF [2019-06-18 10:41:20]

glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase

locus: BSU34620

pI: 5.18

mw: 68.68 kDa

protein length: 589 aa

gene length: 1770 bp

function: starch and maltodextrin utilization

product: glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,555,902 3,557,671

The protein

Protein family

• [SW|glycosyl hydrolase 13 family] (according to Swiss-Prot)

Structure

• [PDB|1J0J] (from G. stearothermophilus, 56% identity, complex with maltotetraose), [PDB|1J0H] (from G. stearothermophilus, 56% identity)

[SW|Localization]

• on both sides of the cytoplasmic membrane and in the periplasm during vegetative growth but in the cytoplasm of prespores [Pubmed|19465663]

Expression and Regulation

Operons

• genes: [gene|EA1BEC9FCFD2A32142A8A8E6D7362DB37E02052A|yvdE]-[gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]-[gene|5771E1976D986638B13ADDE97EDE32709E355923|mdxE]-[gene|EA2DD9892C1128ADCD6C8BC61248EBE45339E6D6|mdxF]-[gene|D72CFFE8ED3EC0280AE92C05A136D66ED1F62251|mdxG]-[gene|68C6FD5D4D9491EE15A5015447959A45C36EA702|yvdJ]-[gene|5F615BB886EC4F7000AAD42258DB8E47616552DE|yvdK]-[gene|6E5AB4620F9A896C32CD77AF314C67035814AE0A|malL]-[gene|CAFAE305B1771DD89E81F75A6713161393BE99A0|pgcM]
  description: [Pubmed|16707683]

  regulation

  • induced in the presence of maltose [Pubmed|9573215]

Biological materials

Mutant

• MGNA-B628 (yvdF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1627 NBRP B. subtilis, Japan]
• BKE34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT, downstream forward: _UP4_TAACATTCTGTTATCGGTAA
• BKK34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT, downstream forward: _UP4_TAACATTCTGTTATCGGTAA

References

• 16707683,19465663,15650689